After the second ligation and third transduction fail, one wonders..
The cell debris that earns a frown
Or P value that gets you down
The ice slurry that melts so fast
That competent cells they float full mast
The Streptococcus so cheery,
That Amp or Kan it makes merry
The enzyme assay that looks all nice
Until the blot satanically dries
The transfer apparatus that stops midway
Even when you remembered to pray
The ligation colony that is not a clone
Transforming ecstatic smile to heartfelt groan
The cells that thrive on a tryptone feast
Except on cross examination they turn out to be yeast
The PCR that only works on alternate weekends,
With prima donna moods that rival fashion trends
I wonder whether all of this, is on ‘entertainment E. coli ’
As a pick me up and comic turn for bored bacilli!
If its being broadcast on some bacterial underground network, I m sure no channel could compete with its TRP's..